Real-time fluorescence detection of exoribonucleases

Real-time fluorescence detection of exoribonucleases.

The identification of RNases or RNase effectors is a continuous challenge, particularly given the current importance of RNAs in the control of genome expression. Here, we show that a fluorogenic RNA-DNA hybrid is a powerful tool for a real-time fluorescence detection and assay of exoribonucleases (RT-FeDEx). This RT-FeDEx assay provides a new strategy for the isolation, purification, and assay ...

Real - Time Multispectral Fluorescence Stand - off Detection

Linda S. Powers National Center for the Design of Molecular Function, Utah State University Research Foundation, and Dept. of Electrical and Computer Engineering, Utah State University, Logan, UT 84322-4155 Thomas D. Wilkerson Dept. of Physics and Center for Atmospheric and Space Science, Utah State University, and Space Dynamics Laboratory, Utah State University Research Foundation, Logan, UT ...

Real-Time Interference Detection in Tracking Loop of GPS Receiver

Global Positioning System (GPS) spoofing could pose a major threat for GPS navigation ‎systems, so the GPS users have to gain a better understanding of the broader implications of ‎GPS.‎ In this paper, a plenary anti-spoofing approach based on correlation is proposed to distinguish spoofing effects. The suggested ‎method can be easily implemented in tracking loop of GPS receiver...

Detection of Congenital Mullerian Anomalies Using Real-Time 3D Sonography

Real-time fluorescence-based detection of furanocoumarin photoadducts of DNA.

Real-time fluorescence detection systems were adapted to identify DNA adducts formed by photogenotoxic phytochemicals. Two assays were developed: the first was based on quantitative polymerase chain reaction (qPCR) while the second used thermal denaturation and renaturation (D-R). Both assays employed yeast DNA, the fluorescent dye SYBR Green and a real-time PCR thermocycler. The furanocoumarin...